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TKPTs were treated with UNC0379 for 1 h ( A – H ) or transfected with <t>siRNA</t> <t>targeting</t> <t>SET8</t> (siSET8) or control siRNA (siCON) (I-M) for 24 h and then exposed to cisplatin for 24 h. B Cells were conducted for TUNEL staining ( A ), and TUNEL (+) cells are shown. DAPI staining was used to localize nuclei in TKPTs. Scale bars = 50 μm. C Cell viability was detected after 24 h by cell counting kit 8 (CCK8). Cell lysates were prepared and subjected to immunoblot analysis with antibodies as indicated ( D , I ). The expression levels of all the proteins were quantified by densitometry. Cleaved cas3 ( E ), PTEN ( F ), and SET8 ( G ) were normalized by GAPDH; H4K20me1 ( H ) was normalized by H4; Cleaved cas3 ( J ), PTEN ( K ), and SET8 ( L ) were normalized by Tubulin; H4K20me1 ( M ) was normalized by H4. Cells were treated as described above. The extracted RNA cell lysates were subjected to quantitative RT-PCR using the primers listed in Supplementary Table . mRNA expression levels of SET and PCNA were normalized relative to mouse β-Actin and analyzed using the 2 −ΔΔCt method ( N – Q ). Data are represented as the mean ± SEM of at least three experiments. * P < 0.05, ** P < 0.01, *** P < 0.001.

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1) Product Images from "SET8 inhibition preserves PTEN to attenuate kidney cell apoptosis in cisplatin nephrotoxicity"

Article Title: SET8 inhibition preserves PTEN to attenuate kidney cell apoptosis in cisplatin nephrotoxicity

Journal: Cell Death & Disease

doi: 10.1038/s41419-025-07526-y

TKPTs were treated with UNC0379 for 1 h ( A – H ) or transfected with siRNA targeting SET8 (siSET8) or control siRNA (siCON) (I-M) for 24 h and then exposed to cisplatin for 24 h. B Cells were conducted for TUNEL staining ( A ), and TUNEL (+) cells are shown. DAPI staining was used to localize nuclei in TKPTs. Scale bars = 50 μm. C Cell viability was detected after 24 h by cell counting kit 8 (CCK8). Cell lysates were prepared and subjected to immunoblot analysis with antibodies as indicated ( D , I ). The expression levels of all the proteins were quantified by densitometry. Cleaved cas3 ( E ), PTEN ( F ), and SET8 ( G ) were normalized by GAPDH; H4K20me1 ( H ) was normalized by H4; Cleaved cas3 ( J ), PTEN ( K ), and SET8 ( L ) were normalized by Tubulin; H4K20me1 ( M ) was normalized by H4. Cells were treated as described above. The extracted RNA cell lysates were subjected to quantitative RT-PCR using the primers listed in Supplementary Table . mRNA expression levels of SET and PCNA were normalized relative to mouse β-Actin and analyzed using the 2 −ΔΔCt method ( N – Q ). Data are represented as the mean ± SEM of at least three experiments. * P < 0.05, ** P < 0.01, *** P < 0.001.

Figure Legend Snippet: TKPTs were treated with UNC0379 for 1 h ( A – H ) or transfected with siRNA targeting SET8 (siSET8) or control siRNA (siCON) (I-M) for 24 h and then exposed to cisplatin for 24 h. B Cells were conducted for TUNEL staining ( A ), and TUNEL (+) cells are shown. DAPI staining was used to localize nuclei in TKPTs. Scale bars = 50 μm. C Cell viability was detected after 24 h by cell counting kit 8 (CCK8). Cell lysates were prepared and subjected to immunoblot analysis with antibodies as indicated ( D , I ). The expression levels of all the proteins were quantified by densitometry. Cleaved cas3 ( E ), PTEN ( F ), and SET8 ( G ) were normalized by GAPDH; H4K20me1 ( H ) was normalized by H4; Cleaved cas3 ( J ), PTEN ( K ), and SET8 ( L ) were normalized by Tubulin; H4K20me1 ( M ) was normalized by H4. Cells were treated as described above. The extracted RNA cell lysates were subjected to quantitative RT-PCR using the primers listed in Supplementary Table . mRNA expression levels of SET and PCNA were normalized relative to mouse β-Actin and analyzed using the 2 −ΔΔCt method ( N – Q ). Data are represented as the mean ± SEM of at least three experiments. * P < 0.05, ** P < 0.01, *** P < 0.001.

Techniques Used: Transfection, Control, TUNEL Assay, Staining, Cell Counting, Western Blot, Expressing, Quantitative RT-PCR

Mice were treated with cisplatin and UNC0379 (UNC) as indicated in Fig. . Kidney tissue lysates were prepared and subjected to immunoblot analysis using antibodies as indicated ( A ). The levels of all the proteins were quantified by densitometry, and γ-H2A, p-p53, and p21 were normalized with H2A, p53, and Tubulin, respectively, ( B ). TKPTs were treated with UNC0379 for 1 h ( C – F ) or transfected with siRNA targeting SET8 (siSET8) or control siRNA (siCON) ( G – J ) for 24 h and then exposed to cisplatin for 24 h. Cell lysates were prepared and subjected to immunoblot analysis using antibodies as indicated ( C , G ). The levels of all the proteins were quantified by densitometry. γ-H2A ( D ), p-p53 ( E ), and p21 ( F ) were normalized with H2A, p53, and GAPDH, respectively, and γ-H2A ( H ), p-p53 ( I ), and p21( J ) were normalized with H2A, p53, and Tubulin, respectively. Data are represented as the mean ± SEM of at least three experiments. * P < 0.05, ** P < 0.01, *** P < 0.001.

Figure Legend Snippet: Mice were treated with cisplatin and UNC0379 (UNC) as indicated in Fig. . Kidney tissue lysates were prepared and subjected to immunoblot analysis using antibodies as indicated ( A ). The levels of all the proteins were quantified by densitometry, and γ-H2A, p-p53, and p21 were normalized with H2A, p53, and Tubulin, respectively, ( B ). TKPTs were treated with UNC0379 for 1 h ( C – F ) or transfected with siRNA targeting SET8 (siSET8) or control siRNA (siCON) ( G – J ) for 24 h and then exposed to cisplatin for 24 h. Cell lysates were prepared and subjected to immunoblot analysis using antibodies as indicated ( C , G ). The levels of all the proteins were quantified by densitometry. γ-H2A ( D ), p-p53 ( E ), and p21 ( F ) were normalized with H2A, p53, and GAPDH, respectively, and γ-H2A ( H ), p-p53 ( I ), and p21( J ) were normalized with H2A, p53, and Tubulin, respectively. Data are represented as the mean ± SEM of at least three experiments. * P < 0.05, ** P < 0.01, *** P < 0.001.

Techniques Used: Western Blot, Transfection, Control

Mice were treated with CP and UNC0379 (UNC) as indicated in ( A ). Kidney tissue lysates were subject to immunoblot analysis using antibodies against Atg5, Beclin-1, LC3-I/LC3-II, Charged Multivesicular Body Protein 2A (CHMP2A), GAPDH. The levels of all those proteins were quantified by densitometry and individually normalized with GAPDH ( B ). TKPTs were pretreated with UNC0379 for 1 h ( C , D ) or transfected with control siRNA or SET8 siRNA for 24 h ( E , F ) and then exposed to CP for 24 h; cell lysates were prepared and subjected to immunoblot analysis using antibodies as indicated ( C , E ). The levels of all the proteins were quantified by densitometry and then individually normalized with Tubulin ( D , F ). TKPTs were transfected with LC3B-GFP expression plasmid and then treated with CP for 24 h in the presence or absence of UNC ( G , H ). DAPI staining was used to localize nuclei in TKPTs. The number of LC3B-GFP puncta per field was quantified ( H ). Scale bars = 5 μm. Data are represented as the mean ± SEM of at least three experiments. * P < 0.05, ** P < 0.01, *** P < 0.001.

Figure Legend Snippet: Mice were treated with CP and UNC0379 (UNC) as indicated in ( A ). Kidney tissue lysates were subject to immunoblot analysis using antibodies against Atg5, Beclin-1, LC3-I/LC3-II, Charged Multivesicular Body Protein 2A (CHMP2A), GAPDH. The levels of all those proteins were quantified by densitometry and individually normalized with GAPDH ( B ). TKPTs were pretreated with UNC0379 for 1 h ( C , D ) or transfected with control siRNA or SET8 siRNA for 24 h ( E , F ) and then exposed to CP for 24 h; cell lysates were prepared and subjected to immunoblot analysis using antibodies as indicated ( C , E ). The levels of all the proteins were quantified by densitometry and then individually normalized with Tubulin ( D , F ). TKPTs were transfected with LC3B-GFP expression plasmid and then treated with CP for 24 h in the presence or absence of UNC ( G , H ). DAPI staining was used to localize nuclei in TKPTs. The number of LC3B-GFP puncta per field was quantified ( H ). Scale bars = 5 μm. Data are represented as the mean ± SEM of at least three experiments. * P < 0.05, ** P < 0.01, *** P < 0.001.

Techniques Used: Western Blot, Transfection, Control, Expressing, Plasmid Preparation, Staining

TKPTs were treated with CP for 24 h in the presence or absence of UNC and Bpv as indicated ( A ) or after transfection of siRNA targeting PTEN (siPTEN) or control siRNA (siCON) for 24 h ( H ). Cell lysates were prepared and subjected to immunoblot analysis with antibodies as indicated. Expression levels of all the proteins were quantified by densitometry, and PTEN ( B , I ), SET8 ( C , J ), Cleaved cas3 ( E , L ) and p53 ( G , N ) were normalized with GAPDH, H4K20me1 ( D , K ) was normalized with H4, and p-p53 ( F , M ) was normalized with p53. TKPTs were transfected with PTEN plasmid (PTEN-OE) or empty vector (Vector-NC) and then exposed to CP for an additional 24 h. Cell lysates were prepared and subjected to immunoblot analysis with antibodies as indicated ( O ). Expression levels of all the proteins were quantified by densitometry, and PTEN ( P ), Cleaved cas3 ( T ), SET8 ( U ), p53 ( S ) and H4 ( W ) were normalized with GAPDH; γ-H2A ( Q ), p-p53 ( R ), and H4K20me1 ( V ) were normalized with H2A, p53, and H4, respectively. Data are represented as the mean ± SEM of at least three experiments. * P < 0.05, ** P < 0.01, *** P < 0.001.

Figure Legend Snippet: TKPTs were treated with CP for 24 h in the presence or absence of UNC and Bpv as indicated ( A ) or after transfection of siRNA targeting PTEN (siPTEN) or control siRNA (siCON) for 24 h ( H ). Cell lysates were prepared and subjected to immunoblot analysis with antibodies as indicated. Expression levels of all the proteins were quantified by densitometry, and PTEN ( B , I ), SET8 ( C , J ), Cleaved cas3 ( E , L ) and p53 ( G , N ) were normalized with GAPDH, H4K20me1 ( D , K ) was normalized with H4, and p-p53 ( F , M ) was normalized with p53. TKPTs were transfected with PTEN plasmid (PTEN-OE) or empty vector (Vector-NC) and then exposed to CP for an additional 24 h. Cell lysates were prepared and subjected to immunoblot analysis with antibodies as indicated ( O ). Expression levels of all the proteins were quantified by densitometry, and PTEN ( P ), Cleaved cas3 ( T ), SET8 ( U ), p53 ( S ) and H4 ( W ) were normalized with GAPDH; γ-H2A ( Q ), p-p53 ( R ), and H4K20me1 ( V ) were normalized with H2A, p53, and H4, respectively. Data are represented as the mean ± SEM of at least three experiments. * P < 0.05, ** P < 0.01, *** P < 0.001.

Techniques Used: Transfection, Control, Western Blot, Expressing, Plasmid Preparation

TKPTs were treated with CP for 24 h in the presence or absence of Bpv ( A ) or after transfection of siRNA targeting PTEN (siPTEN) or control siRNA (siCON) for 24 h ( E ). Cell lysates were prepared and subjected to immunoblot analysis using antibodies as indicated ( A , E ). The levels of all the proteins were quantified by densitometry and then individually normalized with GAPDH and LC3-I, respectively ( B – D , F – H ). TKPTs were transfected with PTEN plasmid (PTEN-OE) or empty vector (Vector-NC) for 24 h and then exposed to CP for an additional 24 h ( I ). Cell lysates were prepared and subjected to immunoblot analysis with antibodies as indicated ( I ) and then individually normalized with GAPDH ( J , L ) or LC3I-I ( K ). TKPTs were transfected with the LC3B-GFP plasmid and then treated with CP for 24 h in the presence or absence of UNC ( M , N ). Cells were photographed with a fluorescent microscope ( M ). The number of LC3B-GFP puncta per field was quantified ( N ). Scale bars = 5 μm TKPTs were treated with Bpv ( O , P ) or transfected with PTEN plasmid (PTEN-OE) or empty vector (Vector-NC) ( O – R ) for 24 h and then exposed to CP for an additional 24 h. Cells were conducted for TUNEL staining ( O , Q ), and the number of TUNEL (+) cells per field was counted in at least 10 fields per section ( P , R ). Scale bars = 25 μm. Data are represented as the mean ± SEM of at least three experiments. * P < 0.05, ** P < 0.01, *** P < 0.001.

Figure Legend Snippet: TKPTs were treated with CP for 24 h in the presence or absence of Bpv ( A ) or after transfection of siRNA targeting PTEN (siPTEN) or control siRNA (siCON) for 24 h ( E ). Cell lysates were prepared and subjected to immunoblot analysis using antibodies as indicated ( A , E ). The levels of all the proteins were quantified by densitometry and then individually normalized with GAPDH and LC3-I, respectively ( B – D , F – H ). TKPTs were transfected with PTEN plasmid (PTEN-OE) or empty vector (Vector-NC) for 24 h and then exposed to CP for an additional 24 h ( I ). Cell lysates were prepared and subjected to immunoblot analysis with antibodies as indicated ( I ) and then individually normalized with GAPDH ( J , L ) or LC3I-I ( K ). TKPTs were transfected with the LC3B-GFP plasmid and then treated with CP for 24 h in the presence or absence of UNC ( M , N ). Cells were photographed with a fluorescent microscope ( M ). The number of LC3B-GFP puncta per field was quantified ( N ). Scale bars = 5 μm TKPTs were treated with Bpv ( O , P ) or transfected with PTEN plasmid (PTEN-OE) or empty vector (Vector-NC) ( O – R ) for 24 h and then exposed to CP for an additional 24 h. Cells were conducted for TUNEL staining ( O , Q ), and the number of TUNEL (+) cells per field was counted in at least 10 fields per section ( P , R ). Scale bars = 25 μm. Data are represented as the mean ± SEM of at least three experiments. * P < 0.05, ** P < 0.01, *** P < 0.001.

Techniques Used: Transfection, Control, Western Blot, Plasmid Preparation, Microscopy, TUNEL Assay, Staining

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Journal: Cell Death & Disease

Article Title: SET8 inhibition preserves PTEN to attenuate kidney cell apoptosis in cisplatin nephrotoxicity

doi: 10.1038/s41419-025-07526-y

Figure Lengend Snippet: TKPTs were treated with UNC0379 for 1 h ( A – H ) or transfected with siRNA targeting SET8 (siSET8) or control siRNA (siCON) (I-M) for 24 h and then exposed to cisplatin for 24 h. B Cells were conducted for TUNEL staining ( A ), and TUNEL (+) cells are shown. DAPI staining was used to localize nuclei in TKPTs. Scale bars = 50 μm. C Cell viability was detected after 24 h by cell counting kit 8 (CCK8). Cell lysates were prepared and subjected to immunoblot analysis with antibodies as indicated ( D , I ). The expression levels of all the proteins were quantified by densitometry. Cleaved cas3 ( E ), PTEN ( F ), and SET8 ( G ) were normalized by GAPDH; H4K20me1 ( H ) was normalized by H4; Cleaved cas3 ( J ), PTEN ( K ), and SET8 ( L ) were normalized by Tubulin; H4K20me1 ( M ) was normalized by H4. Cells were treated as described above. The extracted RNA cell lysates were subjected to quantitative RT-PCR using the primers listed in Supplementary Table . mRNA expression levels of SET and PCNA were normalized relative to mouse β-Actin and analyzed using the 2 −ΔΔCt method ( N – Q ). Data are represented as the mean ± SEM of at least three experiments. * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: RIPA lysis buffer (9806) was purchased from Cell Signaling Technology (Danvers, MA, USA). con siRNA (sc-37007), SET8 siRNA (sc-155946), and p53 siRNA (sc-29436) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA).

Techniques: Transfection, Control, TUNEL Assay, Staining, Cell Counting, Western Blot, Expressing, Quantitative RT-PCR

Journal: Cell Death & Disease

Article Title: SET8 inhibition preserves PTEN to attenuate kidney cell apoptosis in cisplatin nephrotoxicity

doi: 10.1038/s41419-025-07526-y

Figure Lengend Snippet: Mice were treated with cisplatin and UNC0379 (UNC) as indicated in Fig. . Kidney tissue lysates were prepared and subjected to immunoblot analysis using antibodies as indicated ( A ). The levels of all the proteins were quantified by densitometry, and γ-H2A, p-p53, and p21 were normalized with H2A, p53, and Tubulin, respectively, ( B ). TKPTs were treated with UNC0379 for 1 h ( C – F ) or transfected with siRNA targeting SET8 (siSET8) or control siRNA (siCON) ( G – J ) for 24 h and then exposed to cisplatin for 24 h. Cell lysates were prepared and subjected to immunoblot analysis using antibodies as indicated ( C , G ). The levels of all the proteins were quantified by densitometry. γ-H2A ( D ), p-p53 ( E ), and p21 ( F ) were normalized with H2A, p53, and GAPDH, respectively, and γ-H2A ( H ), p-p53 ( I ), and p21( J ) were normalized with H2A, p53, and Tubulin, respectively. Data are represented as the mean ± SEM of at least three experiments. * P < 0.05, ** P < 0.01, *** P < 0.001.

Techniques: Western Blot, Transfection, Control

Journal: Cell Death & Disease

Article Title: SET8 inhibition preserves PTEN to attenuate kidney cell apoptosis in cisplatin nephrotoxicity

doi: 10.1038/s41419-025-07526-y

Figure Lengend Snippet: Mice were treated with CP and UNC0379 (UNC) as indicated in ( A ). Kidney tissue lysates were subject to immunoblot analysis using antibodies against Atg5, Beclin-1, LC3-I/LC3-II, Charged Multivesicular Body Protein 2A (CHMP2A), GAPDH. The levels of all those proteins were quantified by densitometry and individually normalized with GAPDH ( B ). TKPTs were pretreated with UNC0379 for 1 h ( C , D ) or transfected with control siRNA or SET8 siRNA for 24 h ( E , F ) and then exposed to CP for 24 h; cell lysates were prepared and subjected to immunoblot analysis using antibodies as indicated ( C , E ). The levels of all the proteins were quantified by densitometry and then individually normalized with Tubulin ( D , F ). TKPTs were transfected with LC3B-GFP expression plasmid and then treated with CP for 24 h in the presence or absence of UNC ( G , H ). DAPI staining was used to localize nuclei in TKPTs. The number of LC3B-GFP puncta per field was quantified ( H ). Scale bars = 5 μm. Data are represented as the mean ± SEM of at least three experiments. * P < 0.05, ** P < 0.01, *** P < 0.001.

Techniques: Western Blot, Transfection, Control, Expressing, Plasmid Preparation, Staining

Journal: Cell Death & Disease

Article Title: SET8 inhibition preserves PTEN to attenuate kidney cell apoptosis in cisplatin nephrotoxicity

doi: 10.1038/s41419-025-07526-y

Figure Lengend Snippet: TKPTs were treated with CP for 24 h in the presence or absence of UNC and Bpv as indicated ( A ) or after transfection of siRNA targeting PTEN (siPTEN) or control siRNA (siCON) for 24 h ( H ). Cell lysates were prepared and subjected to immunoblot analysis with antibodies as indicated. Expression levels of all the proteins were quantified by densitometry, and PTEN ( B , I ), SET8 ( C , J ), Cleaved cas3 ( E , L ) and p53 ( G , N ) were normalized with GAPDH, H4K20me1 ( D , K ) was normalized with H4, and p-p53 ( F , M ) was normalized with p53. TKPTs were transfected with PTEN plasmid (PTEN-OE) or empty vector (Vector-NC) and then exposed to CP for an additional 24 h. Cell lysates were prepared and subjected to immunoblot analysis with antibodies as indicated ( O ). Expression levels of all the proteins were quantified by densitometry, and PTEN ( P ), Cleaved cas3 ( T ), SET8 ( U ), p53 ( S ) and H4 ( W ) were normalized with GAPDH; γ-H2A ( Q ), p-p53 ( R ), and H4K20me1 ( V ) were normalized with H2A, p53, and H4, respectively. Data are represented as the mean ± SEM of at least three experiments. * P < 0.05, ** P < 0.01, *** P < 0.001.

Techniques: Transfection, Control, Western Blot, Expressing, Plasmid Preparation

Journal: Cell Death & Disease

Article Title: SET8 inhibition preserves PTEN to attenuate kidney cell apoptosis in cisplatin nephrotoxicity

doi: 10.1038/s41419-025-07526-y

Figure Lengend Snippet: TKPTs were treated with CP for 24 h in the presence or absence of Bpv ( A ) or after transfection of siRNA targeting PTEN (siPTEN) or control siRNA (siCON) for 24 h ( E ). Cell lysates were prepared and subjected to immunoblot analysis using antibodies as indicated ( A , E ). The levels of all the proteins were quantified by densitometry and then individually normalized with GAPDH and LC3-I, respectively ( B – D , F – H ). TKPTs were transfected with PTEN plasmid (PTEN-OE) or empty vector (Vector-NC) for 24 h and then exposed to CP for an additional 24 h ( I ). Cell lysates were prepared and subjected to immunoblot analysis with antibodies as indicated ( I ) and then individually normalized with GAPDH ( J , L ) or LC3I-I ( K ). TKPTs were transfected with the LC3B-GFP plasmid and then treated with CP for 24 h in the presence or absence of UNC ( M , N ). Cells were photographed with a fluorescent microscope ( M ). The number of LC3B-GFP puncta per field was quantified ( N ). Scale bars = 5 μm TKPTs were treated with Bpv ( O , P ) or transfected with PTEN plasmid (PTEN-OE) or empty vector (Vector-NC) ( O – R ) for 24 h and then exposed to CP for an additional 24 h. Cells were conducted for TUNEL staining ( O , Q ), and the number of TUNEL (+) cells per field was counted in at least 10 fields per section ( P , R ). Scale bars = 25 μm. Data are represented as the mean ± SEM of at least three experiments. * P < 0.05, ** P < 0.01, *** P < 0.001.

Techniques: Transfection, Control, Western Blot, Plasmid Preparation, Microscopy, TUNEL Assay, Staining

Effects of β2-AR knockdown or overexpression on the expression of TNFα in LPS-treated RAW264.7 cells treated with Ad. Cells were transfected with the specific siRNA targeting β2-AR (β2-AR-siRNA) and control siRNA (Con-siRNA), and plasmid overexpressing β2-AR (pcDNA-β2-AR) and its control (pcDNA), respectively. ( a , c ) β2-AR expression was then examined via immunoblotting. ( b , d ) Transfected cells were treated with LPS with or without Ad for 2 h, and TNFα protein levels were determined (upper panel). One representative result from three independent experiments is shown. GAPDH was used as a loading control. The band density was quantitatively analyzed using ImageJ software. The results (lower panel in ( b , d )) are expressed as mean ± SE ( n = 3). *** p < 0.001; # p < 0.05 and ### p < 0.001; ††† p < 0.001.

Journal: Current Issues in Molecular Biology

Article Title: Catecholamines Attenuate LPS-Induced Inflammation through β2 Adrenergic Receptor Activation- and PKA Phosphorylation-Mediated TLR4 Downregulation in Macrophages

doi: 10.3390/cimb46100675

Figure Lengend Snippet: Effects of β2-AR knockdown or overexpression on the expression of TNFα in LPS-treated RAW264.7 cells treated with Ad. Cells were transfected with the specific siRNA targeting β2-AR (β2-AR-siRNA) and control siRNA (Con-siRNA), and plasmid overexpressing β2-AR (pcDNA-β2-AR) and its control (pcDNA), respectively. ( a , c ) β2-AR expression was then examined via immunoblotting. ( b , d ) Transfected cells were treated with LPS with or without Ad for 2 h, and TNFα protein levels were determined (upper panel). One representative result from three independent experiments is shown. GAPDH was used as a loading control. The band density was quantitatively analyzed using ImageJ software. The results (lower panel in ( b , d )) are expressed as mean ± SE ( n = 3). *** p < 0.001; # p < 0.05 and ### p < 0.001; ††† p < 0.001.

Article Snippet: Silencer Select Pre-designed siRNAs for β2-AR (β2-AR-siRNA, ID: s62080) and a control siRNA (Con-siRNA, catalog #4390843) were purchased from Thermo Fisher Scientific Inc. RAW264.7 cells were transfected with 5 nM β2-AR-siRNA and Con-siRNA using Lipofectamine RNAiMAX according to the manufacturer’s protocol [ ].

Techniques: Knockdown, Over Expression, Expressing, Transfection, Control, Plasmid Preparation, Western Blot, Software

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